Bio-Rad DCode™ Universal Mutation Detection System - Manual (Page 11)

To reset the graph to the default settings, select Default Graph Axis from the Graph
menu. WinMelt users can also click on the right mouse button. The temperature range will be
set to 20–120 °C and the sequence range will include the longest sequence in the project.
Fig. 3.8. Edit Graph Axis window.
3.8 Printing the Graph
When the graph is printed the sequence information is also printed. To print the graph, first
ensure that the Graph View window is the active window, then select Print from the File
menu. A standard Print dialog box will appear. If necessary, select any print options and then
click OK. To see what will be printed before printing, use the Print Preview command on the
File menu.
3.9 Exporting a Sequence, Melt Profile data, and Graph
The new mutant sequence can be exported or saved outside the project file for later use.
To save the mutant sequence, select the TP53ex8 mutant sequence in the Project View. Then
select Export from the Sequence menu. A standard file save dialog box will appear. Save the
new sequence as "TP53ex8 mutant". The sequence will be saved in the FASTA format.
The melting profile data for each sequence can be exported or saved for viewing in another
program (e.g. a spreadsheet program). To save the melting profile data for the mutant sequence,
select the TP53ex8 mutant sequence in the Project View. Then select Export data from the
Sequence menu. A standard File Save dialog box will appear. Save the melting profile data
as "TP53ex8 mutant data". The melting profile data will be saved as text file.
The graph can be saved as an image file for later importing into another application. To
save the graph, select Export from the Graph menu. A standard File Save dialog box will
appear. Save the graph as "TP53ex8 graph".
3.10 Saving the Project
It is recommended that the project be saved. When a project is saved, all sequences,
sequence descriptions, and options are stored in the project file. The melting profiles and
graphs are recalculated when the file is reopen. Select Save from the File menu to save the
project. A standard File Save dialog box will appear. Save the project as "TP53ex8 project".
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