Bio-Rad DCode™ Universal Mutation Detection System - Manual (Page 20)

Fig. 7.3. Printed graph and sequence information.
17
DNA melting profile graph
Betaglobin
Sequence position (bp)
Betaglobin exon 1 with GC-clamp
Melting probability: 50%
GGGCTGGGCA
TAAAAGTCAG GGCAGAGCCA TCTATTGCTT ACATTTGCTT 50
CTGACACAAC TGTGTTCACT AGCAACCTCA AACAGACACC ATGGTGCACC 100
TGACTCCTGA GGAGAAGTCT GCCGTTACTG CCCTGTGGGG CAAGGTGAAC 150
GTGGATGAAG TTGGTGGTGA GGCCCTGGGC AGGTTGGTAT CAAGGTTACA 200
AGACAGGTTT AAGGAGACCA ATAGAAACTG GGCATGTGGA GACAGAGAAG 250
ACTCTTGGGT TTCTGATAGG CACTGACTCT CTCTGCCTAT TGGTCTATTC 300
GGGCGGGGGC GGCGGGACGG GCGCGGGGCG CGGCGGGCG 399
Mutant Betaglobin exon 1 with GC-clamp
Melting probability: 50%
GGGCTGGGCA
TAAAAGTCAG GGCAGAGCCA TCTATTGCTT ACATTTGCTT 50
CGGACACAAC TGTGTTCACT AGCAACCTCA AACAGACACC ATGGTGCACC 100
TGACTCCTGA GGAGAAGTCT GCCGTTACTG CCCTGTGGGG CAAGGTGAAC 150
GTGGATGAAG TTGGTGGTGA GGCCCTGGGC AGGTTGGTAT CAAGGTTACA 200
AGACAGGTTT AAGGAGACCA ATAGAAACTG GGCATGTGGA GACAGAGAAG 250
ACTCTTGGGT TTCTGATAGG CACTGACTCT CTCTGCCTAT TGGTCTATTC 300
GGGCGGGGGC GGCGGGACGG GCGCGGGGCG CGGCGGGCG 399
100.0
94.0
88.0
82.0
76.0
70.0
1 69 137 205 273 341