Bio-Rad DCode™ Universal Mutation Detection System - Manual (Page 8)

A sequence can be added to the project by two methods: 1) The sequence can be import-
ed from a text file, or 2) the entire sequence can be typed into the program from the keyboard.
In this tutorial the sequence will be imported into the Project View. Choose Import from the
Sequence menu. A standard open dialog box will appear. Open the text file "TP53ex8 wild-
type" provided with the program. The new sequence will be imported into the Project View
as shown in Figure 3.2. The information displayed in the Project View contains the name of
the sequence, the graphing option, the melting probability, the length of the sequence, and
the beginning portion of the sequence.
Fig. 3.2. Imported TP53ex8 wild-type sequence.
3.3 Duplicating a Sequence
Before any changes are made to the sequence, a duplicate sequence will be created. This
duplicate sequence will be used to create a mutated sequence. Click on the icon in front of the
TP53ex8 wild-type sequence in the Project View, then select Duplicate from the Sequence
menu. A new sequence will appear in the Project View just below the first sequence as shown
in Figure 3.3. The two sequences are identical, except that the new sequence is named "Copy
of TP53ex8 wild-type".
Fig. 3.3. Duplicating the TP53ex8 wild-type sequence.
3.4 Editing a Sequence
A single base mutation will be introduced in the duplicated sequence. Double-click on the
icon in front of the "Copy of TP53ex8 wild-type" sequence or select Display from the
Sequence menu. The Sequence Display window will appear (Figure 3.4).
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