Bio-Rad DCode™ Universal Mutation Detection System User Manual

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Section 5
The Project View

The Project View is a window that shows an overview of the project file (Figure 5.1).

The name of the project is shown in the window title. Each line in the Project View represents
one sequence. For each sequence in the project, the following information is shown:

A small icon for each sequence

The sequence description

Whether or not to show the melting profile graph for this sequence

The melting probability used for melting profile calculations

The sequence length

The initial part of the sequence

Fig. 5.1. The Project View window.

Projects are created, opened, and saved using the commands on the File menu (Section

7.1) or buttons on the toolbar (Section 9). When the Project View is active the sequences in
the project can be printed by selecting Print from the File menu or by pressing the Print
button in the toolbar. If a sequence is selected, only that sequence will be printed. If no
sequences are selected, then all the sequences in the Project View will be printed.

When importing a sequence into the Project View, the text file should contain only the

sequence or conform to the FASTA, GenBank or EMBL flat file formats. Illegal symbols
will be removed and the sequence will be truncated to 10,000 nucleotides if necessary
(9,000 in Windows 3.1x). Characters different from "acgtu and ACGTU" will not be accept-
ed. Both upper and lower case letters can be used. The case of the letters will be preserved
when the sequence is imported. The maximum number of sequences that can be in one pro-
ject is 50.

The sequences and related information are manipulated using the commands in the

Sequence menu. The Sequence menu commands are.

New

Adds a new empty sequence.

Duplicate

Duplicates a selected sequence.

Display

Displays the properties of a selected sequence and allows editing of the
sequence.

Delete

Deletes a selected sequence.

Import

Imports a sequence from a text file in GenBank, EMBL, FASTA or plain
text file format.

Export

Exports a selected sequence to a text file in FASTA format.

Export Data

Exports the melting profile data to a text file for processing with another
application.

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