Bio-Rad Molecular Mass Rulers - Manual (Page 3)

Fig. 1. 5 µl of the EZ Load 20 bp molecular ruler was loaded onto a 2.5%
Amplisize agarose gel. The gel was run at 140 V for 3 hours in 1x TBE
buffer. The gel was stained in EtBr for 15 minutes and destained in dH
2
O for
30 minutes.
3
EZ Load 20 bp Molecular Ruler
Catalog Number 170-8351
Use Load 5 µl per lane. This loading translates into
500 ng of DNA per lane. Adjustments may be
made to the loading volume for different well
sizes and desired band intensity. The EZ Load
20 bp PCR molecular ruler can be resolved in
1.5–2.5% standard agarose gels, Amplisize
agarose gels up to 4%, and polyacrylamide gels
up to 8%.
Contents 1 vial EZ Load 20 bp molecular ruler, 500 µl
supplied in 5% glycerol, 15 mM Tris pH 8.0,
1.5 mM EDTA, 0.04% bromophenol blue,
0.04% xylene cyanole FF.
Concentration 100 µg/ml
Size 50 bands: 20–1,000 bp in exact 20 bp
increments. A visually distinct reference band at
200 bp contains three times the concentration of
material found in the other bands.
Please note The EZ Load 20 bp molecular ruler does not
always resolve well in gels made of pure
NuSieve GTG. Use Bio-Rad Amplisize Agarose,
or the recommended mixture of 3:1 NuSieve
GTG: standard agarose.
2
1,000 bp
2 0 0 b p
2 0 b p
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