Bio-Rad Molecular Mass Rulers - Manual (Page 4)

Fig. 2. 5 µl of the EZ Load 100 bp molecular ruler was loaded onto a
2.5% Amplisize agarose gel. The gel was run at 140 V for 3 hours in
1x TBE buffer. The gel was stained in EtBr for 15 minutes and destained in
d H
2
O for 30 minutes.
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EZ Load 100 bp Molecular Ruler
Catalog Number 170-8352
Use Load 5 µl per lane. This loading translates into
250 ng of DNA per lane. Adjustments may be
made to the loading volume for different well
sizes and desired band intensity. The EZ Load
100 bp molecular ruler can be resolved in
1–2.5% agarose gels and polyacrylamide gels up
to 8%.
Contents 1 vial EZ Load 100 bp molecular ruler, 500 µl
supplied in 5% glycerol, 15 mM Tris pH 8.0,
1.5 mM EDTA, 0.04% bromophenol blue,
0.04% xylene cyanole FF.
Concentration 50 µg/ml
Size 10 bands: 100–1,000 bp in exact 100 bp increments.
Please note The EZ Load 100 bp molecular ruler may show a
double- or triple-banding pattern in poly-
acrylamide gels.
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1,000 bp
1 0 0 b p
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