Bio-Rad Molecular Mass Rulers - Manual (Page 8)

Fig. 6. 5 µl of the EZ Load Precision Molecular Mass Standard was
loaded onto a 1.8% Molecular Biology Certified agarose gel. The gel was
run at 70 V for 75 minutes in 1x TBE buffer. The gel was stained in EtBr for
15 minutes and destained in dH
2
O for 30 minutes.
1 3
EZ Load Precision Molecular Mass
Standard
Catalog Number 170-8356
Use Load 5 µl per lane. Adjustments may be made to
the loading volume for different well sizes and
desired band intensity. The EZ Load Precision
Molecular Mass Standard can be resolved in
agarose gels of up to 2% and polyacrylamide
gels up to 8%.
When used at 5 µl per lane:
1,000 bp
100 ng
700 bp 70 ng
500 bp 50 ng
200 bp 20 ng
100 bp 10 ng
Contents 1 vial EZ Load Precision Molecular Mass
Standard, 500 µl supplied in 5% glycerol, 15 mM
Tris pH 8.0, 1.5 mM EDTA, 0.04% bromophenol
blue, 0.04% xylene cyanole FF.
Concentration 50 µg/ml
Determination The concentration values of the EZ Load
of concentration Precision Molecular Mass Standard fragments
are determined by UV absorbance at 260 nm
wavelength using the conversion factor:
1 . 0 0 A
2 6 0
unit = 50 µg/ml DNA. Fragment
concentrations are accurate to within ± 1%.
Please note To maintain accurate fragment concentrations, it
is imperative to spin down any condensate and
mix contents thoroughly before opening tubes
and withdrawing aliquots.
1 2
1,000 bp
700 bp
500 bp
200 bp
100 bp
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