Bio-Rad Molecular Mass Rulers - Manual (Page 7)

Fig. 5. 5 µl of the EZ Load 1 kb molecular ruler was loaded onto a 0.7%
Molecular Biology Certified agarose gel. The gel was run at 140 V for
2.5 hours in 1x TBE buffer. The gel was stained in EtBr for 15 minutes and
destained in dH
2
O for 30 minutes.
1 1 1 0
EZ Load 1 kb Molecular Ruler
Catalog Number 170-8355
Use Load 5 µl per lane. This loading translates into
400 ng of DNA per lane. Adjustments may be
made to the loading volume for different well
sizes and desired band intensity. The EZ Load
1 kb molecular ruler can be resolved in agarose
gels of up to 2%.
Contents 1 vial EZ Load 1 kb molecular ruler, 500 µl
supplied in 5% glycerol, 15 mM Tris pH 8.0,
1.5 mM EDTA, 0.04% bromophenol blue,
0.04% xylene cyanole FF.
Concentration 80 µg/ml
Size 15 bands: 1–15 kb in exact 1 kb increments. A
visually distinct reference band at 5 kb contains
three times the concentration of material found
in the other bands.
1 5 K b
5 K b
1 K b
4006137b.qxd 09/16/1999 3:22 PM Page 10