Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 104)

Bio-Plex Manager Software 6.1 User Guide | Preparing Protocols
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C ALCULATING C ONCENTRATIONS A UTOMATICALLY
If a dilution series was created for the standards, the software can
automatically calculate the concentrations of each standard.
1. Select the appropriate Most Concentrated Standard well number. (The
most concentrated standard must be in either the first standard well or
the last.)
Figure 83. Entering standard concentrations automatically
2. Enter the concentration of the most concentrated standard in the
Concentration of S1 field and the dilution factor for the remaining
standards in the Dilution Factor field.
NOTE: Calculations are made by dividing by the dilution factor, so do not
enter fractional values for the dilution factor. If each standard is half the
concentration of the previous standard, enter 2 for the dilution factor. If each
standard concentration is one-fourth the previous, enter 4, and so on.
3. Click the Calculate button when all the information is entered. The
concentrations in the table are automatically updated.
If the Apply same concentrations to all analytes checkbox, below the
concentrations table, is selected, the concentration values are applied to all
the analyte standards. Deselect the checkbox if you want to enter different
concentrations for each analyte standard, and repeat the steps.
BioPlex_6.book Page 92 Friday, September 23, 2011 11:55 AM