Bio-Rad Bio-Plex Software® Upgrades and Conversions User Manual
Page 171
Report Table
159
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Conc in Range — observed concentration that falls within the
range of standard concentrations, given a specified recovery
range. Values outside the range are flagged as ***. See page 160
for how Conc in Range relates to the Obs/Exp * 100 Column
•
Obs Conc — observed concentration of the analyte, calculated
from its fluorescent intensity and the standard curve. Note that the
observed concentrations of standards are back-calculated from
their fluorescent intensities and the standard curve
•
Obs Conc Std Dev — observed concentration of the analyte, with
the standard deviation of the replicate group of wells
•
Obs Conc Std Err — observed concentration of the analyte, with
the standard deviation of a replicate group of wells, divided by the
square root of the number of replicates
•
Obs Conc % CV — observed concentration of the analyte, with
coefficient of variation of a replicate group of wells. If this value is
high, you may want to review the individual FI values and select the
appropriate member of the replicate group as an outlier
•
Exp Conc — expected concentration of the analyte standard or
control, as specified in the Enter Standards Info window or Enter
Controls Info window, respectively
•
Obs/Exp * 100 — recovery rate for the observed versus expected
concentrations of the standard or control (see page 160)
•
Group — defined group of the selected well (see page 72)
•
Ratio — a calculation performed when you have grouped samples
in the plate formatting section. This calculation is the ratio of the
member-to-reference measured values, or vice versa. (For more
information, see Ratio (Simple) and the Grouping Function, on
page 226 of the Appendix)
•
Dilution — specified dilution factor of the analyte
•
Bead Count — number of beads counted in region for the analyte
•
Bead Mean — mean fluorescent intensity of the beads counted in
region for the analyte
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Bead Std Dev — standard deviation of the bead mean
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Bead Std Err — standard error of the bead mean
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Bead %CV — coefficient of variation of the bead mean
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Trimmed Mean — bead mean, excluding the top 5% and bottom
5% of fluorescent intensities
•
Trimmed Std Dev — standard deviation of the trimmed mean
•
Trimmed Std Err — standard error of the trimmed mean
•
Trimmed %CV — coefficient of variation of the trimmed mean
•
Sampling Errors — error codes for a reading (see page 105)
BioPlex_6.book Page 159 Friday, September 23, 2011 11:55 AM