Bio-Rad Bio-Plex Pro™ Human Isotyping Assays - Manual (Page 27)

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2. Run Protocol — on the pop-up screen, select Load Plate and click
OK to start acquiring data.
3. Use the Wash Between Plates command after every plate run
to reduce the possibility of clogging the instrument.
4. If acquiring data from more than one plate, empty the waste bottle
and refill the sheath bottle after each plate (if HTF are not present).
Select Wash Between Plates and follow the instructions. Then repeat
the Prepare Protocol and Acquire Data instructions.
5. When data acquisition is complete, select Shut Down and
follow the instructions.
Reacquire Data
It is possible to acquire data from a well or plate a second time using the
Rerun/Recovery mode located below Start in the Run Protocol step.
Any previous data will be overwritten.
1. Check the wells from which data will be reacquired.
2. Aspirate the buffer with the wash method of choice, but do not
perform wash step.
3. Add 100 µl of assay buffer to each well. Cover the plate with a new
sheet of sealing tape.
4. Repeat the Acquire Data steps to reacquire data. The data acquired
should be similar to those acquired initially; however, the acquisition
time will be extended because the wells have fewer beads.
Data Analysis
Quality Controls
If the quality controls were run in the assay plate, open the results (.rbx)
file, click on Report Table, and locate the control wells. Compare the
observed concentrations against the lot-specific control ranges in the
product data sheet.