Bio-Rad Bio-Plex Pro™ Human Isotyping Assays - Manual (Page 9)

7
lmportant Considerations
Instruments and Software
The Bio-Plex Pro
™ assays described in this manual are compatible with
all currently available Luminex-based life science research instruments.
Assays can be read and analyzed with either Bio-Plex Manager
™ software
or Luminex xPoNENT software (section 9).
Assay Procedures
Pay close attention to vortexing, shaking, and incubation times and to
Bio-Plex
® reader PMT (RP1) setting, as these have been optimized
specifically for each assay panel.
Assay Quick Guide
Each assay kit includes a printed Bio-Plex Pro Assay Quick Guide (bulletin
#10028208), which can be used to prepare and run a full 1 x 96-well assay
plate. Users can also download a copy at www.bio-rad.com/bio-plex.
Bead Regions
Bead regions for all analytes are listed in the Read Plate section.
Multiplexing Capabilities
Due to different sample dilution considerations, IgE assay should not be
multiplexed with other assays in the isotyping product line.
Multiplexing singleplex assays (IgA, IgM, and IgG total) is not recommended
due to lot differences of components. If assaying for IgA and IgM, then
consider running the 6-plex panel.
To avoid cross-reactivity, IgG total assay should not be multiplexed with any
of its subclasses (IgG
1
, IgG
2
, IgG
3
, and IgG
4
).
Detailed Instructions
The following pages provide detailed instructions for each step of the
assay procedure, including preparation, running the assay, and reading the
plate with Bio-Plex Manager
™ and Luminex xPoNENT software.