Bio-Rad BioLogic DuoFlow Software User Manual

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The BioLogic DuoFlow version 5.0 software introduces three new features and several
enhancements to BioLogic DuoFlow

version 4.0 while maintaining the familiar intuitive

and easy-to-use design.

New Features

• Scouting Wizard

• Buffer Editor

• Method Templates

Enhanced Features

• Below Threshold Fraction Collection

• Rack & Tube # and Rack & Grid # BioFrac

fraction collector numbering

schemes

• Time and Volume based chromatograms

• New Buffer Blending buffer systems

• Improved High Flow non Buffer Blending feature

• Vertical chromatogram trace shifting in Trace Compare overlay view

• Trace Compare display settings saved

• Enhanced Online Help

New Features

Scouting Wizard

The Scouting Wizard is a feature that automates the setup of scouting experiments.
Scouting is a procedure used to systematically optimize the purification conditions for a
specific target molecule(s) (i.e. protein). Molecules such as proteins differ from one
another in their charge, hydrophobicity, solubility, reactivity, substrate specificity and

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