Bio-Rad BioLogic DuoFlow™ pH Monitor - Manual (Page 2)

Instructions for first time use
During shipment, the air bubble in the electrode’s stem may have moved into the bulb area. If bubbles are seen in
the bulb area, hold the electrode by its top cap and shake downwards, as is done with a clinical thermometer.
Do not expose the electrode to extreme pH solutions for prolonged periods of time, doing so will severely shorten
the lifespan of the electrode.
Before you install the pH electrode in the flow cell hydrate the pH bulb and reference junction by removing the
electrode protector boot and immersing the lower end of the electrode into distilled water or a pH 4.0 buffer for 30
minutes.
Assembling and Installing your pH Monitor
The flow cell is equipped with a mounting bracket for easy attachment to any system. The tilt of the flow cell
inhibits bubble formation.
1. Mount the flow cell to the DuoFlow rack using the mounting bracket. If a UV detector and Conductivity monitor
are being used we recommend attaching them to this same bracket.
2. Plumb the flow cell from its inlet (lower port) to the Conductivity monitor using the orange (0.02” ID) PEEK
tubing supplied with the pH monitor. Connect the outlet port to your fraction collector. Both ports are threaded
for use with ¼-28 fittings. If you anticipate high flow rates that create high backpressure, install the green
(0.03” ID) PEEK tubing.
3. Ensure that the black o-ring is in the flow cell and the red o-ring is on the lower half of the pH electrode.
4. Insert the pH electrode into the top of the flow cell and hand tighten the nut until some
resistance is encountered. Do not over tighten.
5. Attach the BNC connector from the pH electrode to the Maximizer or SIM, see figures 2 and 3.
Plumb the flow cell downstream of any backpressure device since the pH probe has a maximum pressure
rating of 75 psi.
Figure 2. pH Monitor connected via SIM module for BioLogic DuoFlow without a Maximizer system
2
FRONT VIEW
SIGNAL IMPORT MODULE
REAR VIEW
pH ELECTRODE
TO BIOLOGIC CONTROLLER
(HR OR DUO FLOW)
OR
USB BITBUS COMMUNICATOR
pH MONITOR
pH FLOW CELL