Bio-Rad Bio-Scale™ MT High-Resolution Empty Columns - Manual (Page 5)

Bio-Scale MT Column Instruction Manual 1
Section 1
Characteristics of the Bio-Scale MT2, MT5, MT10,
and MT20 Columns
1.1 Introduction
The Bio-Scale MT columns have been designed specifically
to provide high resolution chromatography of bio-molecules
when packed with any suitable chromatography media. Typical
packings might include ion exchange, hydrophobic interaction,
hydroxyapatite, or affinity chromatography media.
The Bio-Scale MT range consists of four sizes (2, 5, 10, and
20 ml), allowing easy scale-up of separation and purification
protocols. The column design has been optimized to provide
easy packing, equilibration, bed-height adjustment, and sample
application. All column parts are bio-compatible for preservation of
sample biological activity.
The Bio-Scale MT column range is designed for use with
Bio-Rad’s BioLogic System, as the column fittings are 1/4-28
Super-flangeless. Adaptors and unions are available from Bio-Rad
for connection of these columns to other medium or high pressure
systems.
1.2 Column Description
Each column consists of a borosilicate glass tube held in place by
two endcaps and a plastic safety shield. The lower bed support
consists of a fixed-position frit and a distribution screen assembly.
Bed height adjustment is provided by the upper bed support,
which is positioned and held in place using a threaded lock-nut.
A removable frit protects the top of the bed and supports a thin
distribution screen which insures efficient sample application to the
bed itself.
Each column can be packed with chromatography media using
either dry or slurry packing methods. A minimum particle size of
5 microns is recommended.