Bio-Rad UNOsphere™ Rapid S Media - Manual (Page 17)

Section 4
Preparing a Cartridge For
Use
Bio-Scale™ Mini UNOsphere™ cartridges contain
20% ethanol v/v as the storage solution. The fully
hydrated support is ready to use after equilibrating
the cartridge in the buffer of choice. To perform a
buffer exchange, connect the cartridge to a liquid
chromatography system or peristaltic pump and
condition it as instructed below:
1. Set pump flow rate to 3.0 ml/min (731 cm/hr) for
the 1 ml cartridge or 6.0 ml/min (288 cm/hr) for
the 5 ml cartridge.
2. Wash the cartridge with degassed low-salt buffer
for 2 min.
3. Wash the cartridge with degassed high-salt
buffer for 5 min.
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