Bio-Rad UNOsphere™ Rapid S Media - Manual (Page 19)

Table 1 Products for Buffer Exchange.
Sample Recommended
Volume
Product Use Catalog #
50–100 µl Bio-Spin 6 column Desalting proteins 732-6000
6 kD
50–100 µl Bio-Spin 30 column Desalting proteins 732-6004
30 kD
100 µl–3 ml Bio-Scale Mini P6 Desalting proteins 732-4502
cartridge 6 kD
Up to 3 ml Econo-Pac 10DG Desalting proteins 732-2010
desalting columns 6 kD
Unlimited Bio-Gel P-6DG gel Desalting proteins 150-0738
6 kD
4.2 General Purification Protocol
Ion exchange chromatography is usually performed
using increasing salt gradients or pH gradients to
elute the sample components. For best results, and
increased cartridge life, samples and buffers should
be degassed and filtered through a 0.45 µm filter.
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