Bio-Rad UNOsphere™ Rapid S Media - Manual (Page 22)

Table 3 Common Buffers for Ion Exchange
Chromatography. 1,2,3
Type of Buffering
Cation Ion Exchanger Buffer Range
Acetic acid 4.8–5.2
Citric acid 4.2–5.2
HEPES 7.6–8.2
Lactic acid
3.6–4.3
MES 5.5–6.7
MOPS 6.5–7.9
Phosphate 6.7–7.6
PIPES 6.1–7.5
Pivalic acid
4.7–5.4
TES 7.2–7.8
Tricine 7.8–8.9
Anion
Bicine 7.6–9.0
Bis-Tris 5.8–7.2
Diethanolamine 8.4–8.8
Diethylamine 9.5–11.5
L-Histidine
5.5–6.0
Imidazole 6.6–7.1
Pyridine 4.9–5.6
Tricine 7.8–8.9
Triethanolamine 7.3–8.0
Tris 7.5–8.0
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