3
Eluent volume
a. Bio-Sil SEC 125 column
Eluent volume
b. Bio-Sil SEC 250 column
Eluent volume
c. Bio-Sil SEC 400 column
Fig. 1. Separation of standard on HPLC size exclusion columns.
A
280
A
280
A
280
C
E
F
A
B
A&B
C D
E
F
A&B
C
D
E
F
D
Lit102E.qxd 09/13/2002 2:35 PM Page 3