Bio-Rad Chromatography Standards User Manual
Page 6
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![background image](/manuals/600454/6/background.png)
Fig. 1.
a. Bio-Sil SEC 125 column (exclusion limit*
≈
80,000).
b. Bio-Sil SEC 250 column (exclusion limit
≈
300,000).
c. Bio-Sil SEC 400 column (exclusion limit
≈
800,000).
Peaks:
A. Protein aggregates (void peak)
B. Thyroglobulin
C.
γ
-globulin
D. Ovalbumin
E. Myoglobin
F. Vitamin B
12
Conditions
Buffer
0.05 M Na
2
HPO
4
, 0.05 M NaH
2
PO
4
,
0.15 M NaCl, pH 6.8
Column
300 x 7.8 mm
Flow rate
1.0 ml/min
Sample
20 µl
Chart speed
1 cm/min
Detection
UV @ 280 nm; 1.0 AUFS
*Nominal exclusion limits given for globular proteins.
4
Lit102E.qxd 09/13/2002 2:35 PM Page 4
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