Bio-Rad Quantum Prep Plasmid Kits - Manual (Page 5)

Section 1
Introduction
1.1 Overview
The original alkaline lysis method for purifying plasmid DNA
from bacterial cultures requires organic reagents and time-
consuming steps to obtain high-quality DNA. The Quantum Prep
plasmid miniprep kit has been optimized for the rapid purification
of high-quality, high-yield plasmid DNA from 1–2 ml liquid
cultures. This kit uses the silicon dioxide exoskeleton of diatoms
as the DNA binding matrix.
1 The advantages of this porous
substrate include ease of resuspension, high affinity for DNA,
simple and efficient processing, elution in deionized water, and an
inherently large surface-to-volume ratio. All of these properties
contribute to the highest purity and yield of DNA. Plasmid DNA
purified with the Quantum Prep kit can be used directly for
fluorescence sequencing, cell transfection, electroporation, and
enzymatic restriction and modification.
1