Bio-Rad Quantum Prep Plasmid Kits - Manual (Page 9)

should become viscous and slightly clear if cell lysis has
occurred.
4. Add 250 µl of the neutralization solution and mix by gently
inverting the capped tube about ten times (do not vortex). A
visible precipitate should form.
5. Pellet the cell debris for 5 mins in a microcentrifuge. A
compact white debris pellet will form along the side or at the
bottom of the tube. The supernatant (cleared lysate) at this
step contains the plasmid DNA.
6. While waiting for the centrifugation step at step 5, insert a spin
filter into one of the 2 ml microcentrifuge wash tubes supplied
with the kit. Mix the Quantum Prep matrix by vortexing or
repeated shaking and inversion of the bottle to insure that it is
completely suspended.
7. Transfer the cleared lysate (supernatant) from step 5 to a spin
filter, add 200 µl of thoroughly suspended Quantum Prep
matrix, then pipet up and down to mix. If you have multiple
samples, transfer the lysates first, then add matrix and mix.
When matrix has been added to all samples and mixed,
centrifuge for 30 secs.
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