Bio-Rad Aurum™ Plasmid Mini Purification Module - Manual (Page 16)

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Problem Possible Cause Possible Solution
More than one band Presence of multimers – Try different host or
on analytical gel typical and variable growth conditions
depending upon plasmid,
bacterial host, etc.
Nicking due to Use endA- host such as
endonuclease activity JM109, DH5
a, or
XL1-Blue
Prolonged exposure to Add neutralization solution
alkaline conditions within 5 min after
addition of lysis solution
Plasmid nicked/ Use of endA+ bacterial Use endA- host such as
degraded host JM109, DH5
a, or
XL1-Blue
Plasmid prep Plasmid nicked, degraded Check integrity of plasmid
performs poorly in or denatured on an analytical gel
enzymatic reactions
Use endA- host
Section 8
Ordering Information
Catalog # Description
732-6470 Aurum Vacuum Manifold, includes column adaptor plate,
4 replacement luer caps, A and B stages, waste collection
tray, vacuum regulator and gauge, tubing, protocol overview,
and instruction manual
732-6400 Aurum Plasmid Mini Kit, 100 preps, includes 100
plasmid binding columns, 100 capless collection tubes (2.0
ml), reagents, protocol overview, and instruction manual