Bio-Rad Aurum™ Plasmid Mini Purification Module - Manual (Page 4)

Section 1
Introduction
The new Aurum plasmid mini kit is optimized for the purification of up to 20 µg
of plasmid DNA, rapidly and inexpensively, without the use of toxic reagents or
alcohol precipitations. The use of membranes to bind and purify plasmid DNA,
in combination with an optimized column design, minimizes handling and
allows plasmid purification to be carried out in either a vacuum or spin format.
In either format, the final elution is carried out in a microcentrifuge. Plasmid
DNA purified with the Aurum plasmid mini kit is eluted into a small volume of
aqueous buffer and is free of salts, bacterial chromosomal DNA, and RNA.
The exceptional purity of the plasmid produced by this system makes it ideal
for use in automated fluorescent sequencing and in any other molecular
biology application.
Section 2
Kit Components
The Aurum plasmid mini kit contains the following components:
resuspension solution 25 ml
lysis solution 25 ml
neutralization solution 40 ml
wash solution 25 ml (5x concentrate)
elution solution 16 ml
plasmid mini columns 100
2 ml capless wash tubes 100
The solutions are specifically formulated for the Aurum plasmid mini kit. They
are NOT interchangeable with solutions used in other kits or protocols.
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