Bio-Rad trUView Cuvettes - Manual (Page 3)

For most spectrophotometers, use the following protocol to ensure the most
accurate assay results.
1. Ensure that the two optical windows of the cuvette are clean and free from
scratches.
2. Determine the baseline absorbance (blank) using a trUView cuvette. For
the most accurate results, be sure to note the orientation of the cuvette
(marked with an arrow at the top of the cuvette) and use a buffer that is
identical to that used with the sample.
3. Insert the trUView cuvette with 50 µl of sample into the cuvette chamber in
the same orientation as used when determining baseline absorbance.
Other Manufacturers
Instruments with a light path height of 15 mm will require the use of an
optional adaptor (170-2512). The height adaptor is inserted into the cell holder
of the spectrophotometer prior to the assay. After the adaptor has been inserted,
the standard protocol may be followed.
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many other types of plastic absorb light at wavelengths below 400 nm, the
trUView cuvette maintains excellent transmission of light at 260 nm, permitting
quantitation of nucleic acids.
The trUView cuvette is compatible with most spectrophotometers and no height
adaptor is required for those instruments with a light path height (z-dimension) of
8.5 mm. A height adaptor is available from Bio-Rad for use with
spectrophotometers with light path heights of 15 mm. Consult your spectrophotometer's
user manual for z-dimension information.
Use the trUView cuvette to completely replace quartz cuvettes or as a
supplement to their use when working with precious DNA or RNA samples.
These cuvettes are certified DNase and RNase free, so the sample may be
recovered from the cuvette and used for additional assays. Sample volumes as
low as 50 µl may be used.
Operating Instructions
trUView cuvettes are compatible with spectrophotometers having a light-path
height (z-dimension) of 8.5 mm. Consult your spectrophotometer's user manual
for z-dimension information.
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