Bio-Rad trUView Cuvettes - Manual (Page 4)

Technical Specifications
Outer Dimensions 12.5 x 12.5 x 45 mm
Light Path Height 8.5 mm
Pathlength 10 mm
Functional Wavelength Range 220–1,100 nm
Baseline Absorbance < 0.3 AU @ 260 nm
Variability < 15 mAU @ 260 nm
Functional Volume > 50 µl
Chemical Compatibility trUView cuvettes are resistant to all
chemical buffers typically used in most life
science laboratories. The trUView cuvettes
are not compatible with some solvents
such as chloroform and toluene.
Contaminants All trUView cuvettes are guaranteed
RNase and DNase free.
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Troubleshooting
Inaccurate Assay Values – Ensure blank and sample are assayed using
the same cuvette orientation.
– For best results measure the blank and sample
in the same trUView cuvette.
– Inspect the cuvette for scratches or dirt on
optical windows.
– Ensure the light path height (z-dimension) of the
spectrophotometer is 8.5 mm. If it is 15 mm,
use the optional height adaptor.
– If cuvette has been reused, discard and use a
new cuvette. Multiple assays will degrade light
transmission properties of the cuvette.
– Visually check the sample has no air bubbles.
Air bubbles can be removed by gently tapping
the cuvette.
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