Bio-Rad Nuvia™ Q Media - Manual (Page 10)

Effect of Conductivity on human IgG Binding Capacity
0
20
40
60
80
100
120
0 2 4 6 8 10 12 14 16
Conductivity, ms/cm
B
in
d
in
g
c
ap
ac
it
y
@
1
0%
B
T
,
m
g
/m
l
Figure 4 shows how binding capacity for human IgG can be achieved
at higher conductivity and lower load buffer pH.
Fig. 4. Effect of conductivity on polyclonal h.IgG binding capacity of Nuvia S media
at pH 4.5. Column: 0.7 x 5.5 cm; flow rate: 2.0 ml/min (300 cm/hr);
sample:
4.5 mg/ml human IgG in 20 mM sodium acetate, pH 4.5 .
6