Bio-Rad Nuvia™ Q Media - Manual (Page 9)

Section 6 : Operation and Maintenance
Nuvia ™ media are designed to achieve the highest productivity (grams of
drug per operational hour per liter of media) possible. Nuvia media should
be run at the highest linear velocities and loading capacities allowed by
the column and the chromatography system. A linear flow rate of 300 cm/
hr and a 20 cm bed is a recommended starting point. Purification may be
optimized by changing the pH, flow rate, or ionic strength of the elution
buffer, modifying the gradient profile, or experimenting with different buffer
salts.
Note: Due to the high binding capacity of Nuvia media, it is
recommended to elute the biomolecule at flow rates <150 cm/hr
to minimize viscosity induced backpressure.
Figure 1 shows the effect of flow rate on backpressure.
Fig. 1. Nuvia S media pressure/flow performance for a 20 cm diameter x 20 cm
bed height column packed to compression factor of 1.17
.
4
Figure 2 shows the effect of conductivity on Nuvia S media binding capacity.
Fig 2. Effect of conductivity on Nuvia S media binding capacity at pH 5.0.
Column: 1.1 x 9.3 cm; flow rate: 9.75 ml/min (600 cm/hr); sample: 5 mg/ml
human IgG or 5 mg/ml conalbumin
.
Figure 3 shows how the effect of conductivity on Nuvia Q media
binding capacity.
5
Effect of Conductivity on Nuvia S Binding Capacity
0
20
40
60
80
100
120
140
160
0 2 4 6 8 10
Conductivity, ms/cm
B
in
di
ng
c
ap
ac
it
y
@
1
0%
B
T
,
m
g/
m
l
human IgG BC @ 10% BT
Conalbumin BC @ 10% BT
Effect of Conductivity on Nuvia Q Binding Capacity
0
20
40
60
80
100
120
140
160
180
200
0 5 10 15 20 25
Conductivity, ms/cm
Bi
nd
in
g
ca
pa
ci
ty
@
1
0%
B
T,
m
g/
m
l