Bio-Rad Nuvia™ Q Media - Manual (Page 11)

Figure 4 shows how binding capacity for human IgG can be achieved
at higher conductivity and lower load buffer pH.
All buffers commonly used for ion exchange chromatography can be
used with Nuvia media (see Table 2). The use of buffering ions that have
the same charge as the functional group on the ion exchanger will
produce the best results.
Table 2. Common buffers for ion exchange chromatography.
Buffer Buffering Range
Nuvia S
Acetic acid
4.8–5.2
Citric acid 4.2–5.2
HEPES 6.8–8.2
Lactic acid
3.6–4.3
MES 5.5–6.7
MOPS 6.5–7.9
Phosphate 6.7–7.6
PIPES 6.1–7.5
TES 6.8–8.2
Tricine 7.8–8.9
Nuvia Q
Bicine 7.6–9.0
Bis-Tris 5.8–7.2
Diethanolamine 8.4–8.8
Diethylamine 9.5–11.5
L-histidine 5.5–6.0
Imidazole 6.6–7.1
Pyridine 4.9–5.6
Tricine 7.4–8.8
Triethanolamine 7.3–8.3
Tris 7.5–8.0
Section 7 : Regeneration and Sanitation
After each run, the packed bed should be washed with 2–6 bed volumes
of 1–2 M NaCl or until absorbance returns to baseline to remove reversibly
bound material. The column can then be sanitized in 1.0 N NaOH at 50–
100 cm/hr; a minimum contact time of 40 min is recommended.
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