Bio-Rad Nuvia™ Q Media - Manual (Page 5)

Section 1: Introduction
Nuvia™ S and Q high-capacity ion exchange media are new ultra high
binding capacity chromatography media designed for any stage in the
purification process from capture to final polish. Their robust physical
properties, high productivity, and exceptional selectivity meet the needs for
biomolecule purification at both laboratory scale and biopharmaceutical
process scale. Nuvia media are designed for the separation of proteins,
nucleic acids, viruses, plasmids, and other macromolecules. Nuvia media
offer high binding capacity at fast linear flow rates while maintaining low
backpressure. The unique properties of Nuvia media position them as
highly effective media for biomolecule purification.
If you have questions about Nuvia media, contact either your local Bio-
Rad process chromatography sales representative or the Bio-Rad
chromatography Technical Support department for further assistance at
1-510-741-6563.
Section 2 : Technical Description
Table 1. Characteristics of Nuvia™ media.
Nuvia S Nuvia Q
Type of ion exchanger Strong cation Strong anion
Functional group –SO3- –N(CH3)3+
Total ionic capacity 90–150 µeq/ml 100-170 µeq/ml
Dynamic binding capacity* 300 cm/hr >110 mg/ml 300 cm/hr >170 mg/ml
Shipping counterion Na+ Cl-
Median particle size 85 ± 15 µm 85 ± 15 µm
Recommended linear flow rate range 50–600 cm/hr 50–600 cm/hr
Chemical stability
1.0 N NaOH (20°C)
up to 1 week up to 1 week
0.1 N NaOH (20°C)** up to 5 yr —
0.01 N NaOH (20°C)** — up to 5 yr
Gel bed compression ratio 1.15–1.18
(settled bed 1.10–1.15
volume/packed bed volume)
pH stability**
2–14 short term 2–14 short term
4–13 long term 4–12 long term
Shipping solution
20% ethanol + 0.1 M NaCl 20% ethanol + 1 M NaCl
Regeneration 1–2 M NaCl 1–2 M NaCl
Sanitation 0.5–1.0 N NaOH 0.5–1.0 N NaOH
Storage conditions 20% ethanol or 0.1 N NaOH 20% ethanol or 0.01 N NaOH
* 10% breakthrough capacity determined with 4.5 mg/ml human IgG in 40 mM NaOAc + 30 mM
NaCl, pH 5.0 for Nuvia S and 5 mg/ml BSA in 20 mM Tris-HCI pH 8.5 for Nuvia Q.
**Data derived under accelerated conditions at 60°C.
NOTE: Recommended elution flow rates are <150 cm/hr to minimize viscosity induced
backpressure.
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