Bio-Rad Nuvia™ cPrime™ Media - Manual (Page 13)

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Section 7
Sanitization and Regeneration
After each chromatography run, the packed media bed should
be washed to remove reversibly bound material and prepare the
column for the subsequent run. This cleaning process is achieved
by washing the column with 2–6 column volumes of 1–2 M
NaCl followed by 2–6 column volumes of 0.1 N NaOH to remove
remaining proteinaceous impurities. Washing should be conducted
until absorbance returns to baseline. The column is now ready to
be sanitized in 1.0 N NaOH.
After sanitization, to equilibrate the column we recommend
applying 4–6 column volumes of a solution such as 60 mM NaOAC
(sodium acetate)
Note: if the column no longer yields reproducible results, the
media may require additional cleaning to remove strongly bound
contaminants. Acceptable cleaning agents include 25% acetic
acid, 8 M urea, 1% Triton X-100, 6 M potassium thiocyanate,
70% ethanol, 30% isopropyl alcohol, 1 N NaOH, or 6 M guanidine
hydrochloride.