Bio-Rad Nuvia™ cPrime™ Media - Manual (Page 9)

6
Buffers
All buffers commonly used for ion exchange chromatography can
be used with Nuvia cPrime media.
Table 2. Common buffers for cation exchange
chromatography. A buffer concentration of 60 mM is
recommended for most buffers.
Buffer Buffering Range
Acetic acid 4.8–5.2
Citric acid 4.2–5.2
HEPES 6.8–8.2
Lactic acid 3.6–4.3
MES 5.5–6.7
Phosphate 6.5–8.0
Tris 7.5–9.0