Bio-Rad Nuvia™ cPrime™ Media - Manual (Page 5)

2
Section 2
Technical Description
Table 1. Nuvia™ cPrime™ media technical description.
Functional group Hydrophobic cation exchanger
Base matrix composition Macroporous highly crosslinked
hydrophilic polymer
Particle size 70 µm ± 10 µm
Dynamic binding capacity* ≥ 40 mg/ml
Ligand density 55–75 µeq/ml
Recommended linear flow
rate range 50–600 cm/hr
Pressure vs. flow
performance** Under 2 bar @ a flow rate of 600
cm/hr
pH stability 2–14 short term
3–13 long term
Chemical stability 1.0 N NaOH, 1.0 N HCl, 25%
HOAc, 8 M Urea, 6 M Gu-HCl,
6 M KSCN, 3 M NaCl, 1% Triton
X-100, 2% SDS + 0.25 M NaCl,
20% ethanol, 70% ethanol, 30%
isopropanol
Shipping solution 20% ethanol
Storage conditions 20% ethanol
Shelf life*** 5 yrs
* at 10% breakthrough hIgG.
** 20 cm X 20 cm packed bed (1.17 compression factor).
*** Stored at room temperature in 20% ethanol under accelarated
conditions.