Bio-Rad PV92 PCR Informatics Kit - Manual

1. Label one 1.5 ml micro test tube with your
initials. Label one screwcap tube containing
200 µl of InstaGene matrix with your initials.
2. Obtain a cup containing saline solution from
your instructor. Pour the saline into your mouth
and rinse vigorously for 30 seconds. Expel the
saline back into the cup.
3. Transfer 1 ml of your saline rinse into the micro
test tube (NOT the screwcap tube) with your
initials. If a P-1000 micropipet is not available,
carefully pour ~1 ml of your saline rinse into
your micro test tube (use the graduations on the
side of the micro test tube to estimate 1 ml).
4. Spin your tube in a balanced centrifuge at full
speed for 2 minutes. When the centrifuge has
completely stopped, remove your tube. You
should see a match-head sized pellet of whitish
cells at the bottom of the tube. If you don’t see
a pellet of this size, decant the saline, refill your
tube with more of your oral rinse, and repeat the
spin.
5. After pelleting your cells, pour off the saline.
Being careful not to lose your pellet, blot your
tube briefly on a paper towel or tissue. It’s OK
for a small amount of saline (< 50 µl, about the
same size as your pellet) to remain in the
bottom of the tube.
6. Resuspend the pellet by vortexing or flicking the
tube so that no clumps of cells remain.
7. Using a 2–20 µl adjustable-volume micropipet
set to 20 µl, transfer all of your resuspended
cells to the screwcap tube containing
InstaGene.
8. Screw the cap tightly on the tube. Shake or
vortex to mix the tube contents.
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Quick Guide
Lesson 1 Cheek Cell DNA Template Preparation
Centrifuge