Bio-Rad PV92 PCR Informatics Kit - Manual (Page 2)

9. When all members of your team have
collected their samples, place the tubes in the
foam micro test-tube holder, and incubate at
56°C for 10 minutes in a water bath. At the
halfway point (5 minutes), shake or vortex the
tubes gently, then place back in the 56°C water
bath for the remaining 5 minutes.
10. Remove the tubes, shake or vortex, and place
the tubes in a boiling water bath (100°C).
Incubate at 100°C for 5 minutes.
11. Remove the tubes from the boiling water bath
and shake or vortex the contents to resuspend.
Pellet the matrix by spinning at 6,000 x g for
5 minutes (or 2,000 x g for 10 minutes) in a
centrifuge.
12. Store your screwcap tube in the refrigerator until
the next laboratory period (or proceed to step 2
of Lesson 2).
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Centrifuge
56°C, 10 min
100°C, 5 min
Water bath
Water bath