Bio-Rad Comparative Proteomics Kit II: Western Blot Module - Manual (Page 2)

5. Slide gel cassette, buffer dam, and
electrode assembly into the clamping
frame.
6. Press down the electrode assembly while
closing the two cam levers of the
clamping frame.
7. Lower the inner chamber into the mini
tank.
8. Completely fill the inner chamber with 1x
TGS gel running buffer, making sure
the buffer covers the short plate (~150 ml).
9. Fill mini tank approximately 200 ml of
1x TGS gel running buffer.
10. Plate sample loading guide on top of the
electrode assembly.
26
Quick Guide
Q
UICK GUIDE
Sample
Loading
Guide
Pipet Tip