Bio-Rad Comparative Proteomics Kit II: Western Blot Module - Manual (Page 6)

Q
UICK GUIDE
Quick Guide
6. Set up the Mini Trans-Blot module with the
black side of the cassette next to the black
side of the Mini Trans-Blot module. Add a
frozen Bio-Ice module and fill with blotting
buffer up to the white clip.
7. Place lid on tank, matching the power
cords red-to-red and black-to-black, then
blot at 20 V for 2.5 hours.
8. At this point the blots can be stored in the
tanks submerged in blotting buffer at room
temperature overnight or the sandwiches
dismantled and the blots placed in blocker
overnight at 4°C.
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