Shellab SRI20PF - Manual (Page 21)

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OPERATION (CONTINUED)
C ALIBRATE THE T EMPERATURE D ISPLAY
Allow the incubator at least 24 hours to stabilize at its operational set point before performing a
calibration. Always use a certified temperature sensing reference device that is regularly calibrated
to 0.1°C by a third party to conduct temperature verifications or calibrations. For best results use a
remote sensing device.
Part 1: Calibrate the Day Time Temperature
1. Place the incubator Manual Mode, with the Day Time Temperature indicator LED
illuminated.
2. Place the temperature sensor of a calibrated reference device inside the incubator as close
as possible to the chamber’s geometric center.
Note: A thermocouple sensor probe’s sleeve may be taped to the shelving, as long as the exposed
copper end is 2 inches (5cm) above the shelf. An exposed sensor probe in direct contact
with the shelving may experience heat sinking, which can result in an inaccurate temperature
reading.
3. Allow the temperature to re-stabilize after closing the door. The reference device ’s
temperature measurement should not change for at least one (1) hour after a significant
disruption for the chamber to be considered stabilized.
4. If the reference device’s and the incubator’s temperature readings are the same, or the
difference between the two falls within the acceptable range of your laboratory protocol
after one (1) hour of stability, the incubator is calibrated.
5. If there is a difference between the two (2) readings, and that offset falls outside your
laboratory protocol ’s acceptable range, adjust the incubator’s temperature display to match
the reference device’s reading. See the next step. If the door was briefly opened to take a
reading, wait fifteen minutes for the temperature to stabilize before correcting for the offset.
6. Press and hold both the UP and DOWN arrows simultaneously. The Temperature Display
will begin flashing and display the current temperature value. Adjust the current value
until it matches the reference device’s temperature reading. This will enter the offset into
the unit’s temperature controller board.
7. After matching the unit’s current value to that of the reference device, wait five seconds.
The incubator’s Temperature Display will cease flashing, and the incubator will now begin
heating or cooling to compensate for the entered temperature offset.
8. Allow the incubator one (1) hour to stabilize after reaching the new temperature.
9. Compare the reference device’s reading with the incubator’s. If the reference device’s and
the incubator’s temperature readings are the same or fall within the range of your
laboratory protocol, the incubator is now calibrated.
10. If the two readings are not the same or fall outside your laboratory protocol, repeat steps 5
– 8.
11. If the temperature readings of the incubator and the reference device still fall outside your
laboratory protocol after three calibration attempts, contact Sheldon Technical Support
for assistance.