Decontamination, Determining when to replace hepa filters, How to install new hepa filters – Labconco XPert Weigh Boxes 5220331 User Manual

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Chapter 6: Maintaining Your Glove Box

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Decontamination

When used in conjunction with microbiologicals, the glove box should be
decontaminated with formaldehyde gas before:

• Performing maintenance work in contaminated areas
• HEPA filter changes
• Moving the glove box to a new location
• Changing research programs

The procedures for performing a gaseous decontamination are thoroughly
outlined in the U.S. Department of Health, Education and Welfare booklet
entitled Formaldehyde Decontamination of Laminar Flow Biological Safety
Cabinets
, available from NIH, Division of Safety, Bethesda, MD 20892, call
301-496-2801.

Determining When to Replace HEPA Filters

The HEPA filters in the filtered glove boxes gradually accumulate airborne
particulate matter and powders from the enclosure and room. The rate of
accumulation will depend upon the cleanliness of the room air, the amount of
time the enclosure is operating, and the nature of work being done in the
enclosure. In typical installations and usage, the HEPA filters will last two to
five years before requiring replacement. Replace HEPA filters if the speed
control is adjusted to full speed or if the normal operating speed and/or static
pressure is double the original starting point. Replace HEPA filters if it fails
the HEPA Filter Leak Test in Chapter 6.

How to Install New HEPA Filters

The XPert Weigh Boxes design has a true bag-in/bag-out filter disposal system
for safely removing the HEPA filter and protecting the worker from toxic
powders and particulates (see Figure 6-1, Filter Changing Diagram). The
accessory bags are not included and can be ordered separately from Labconco
(see Chapter 7). Two bags will be required for each HEPA filter for a total of 4
each to complete the change process. For your safety, wear appropriate
personal protective equipment during the change of HEPA filters. If working
with micro-biologicals, use the decontamination procedure referenced above.
(Consult your Safety Officer before performing filter changes).

1. Turn the glove box OFF. Refer to Appendix A for replacement parts

and Appendix B for dimensions.

2. Loosen the six filter nuts that secure each HEPA filter.
3. Place the accessory bag over the HEPA filter and seal to the filter

housing.

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