UVP 81-0357-01 PCR Hoods - Manual (Page 13)

PCR Workstations and Cabinets Page 13
Decontaminated UV airflow into and out
of the chamber
The Makrolon ® door is UV blocking and will not allow UV radiation to pass through the door. The
germicidal tubes will shut off if the door is opened. The PCR Workstation contains a powerful source of
UV radiation that will cause damage to unprotected eyes and skin. Before operating any unit, be sure all
personnel in the area are properly protected. Even though the unit shuts the UV off when the door is
open, UV Blocking Eyewear should be worn as well. UVP has a complete line of UV Blocking Eyewear,
including Spectacles, Goggles and Faceshield, designed for this purpose.
Extended periods of UV exposure can be used to disinfect the interior chamber by the destruction of
organisms. Refer to the Maintenance section for information on measuring the intensity of the UV tubes
with a UV meter.
NOTE: Do not attempt to perform PCR procedures with the germicidal lamp on. The germicidal
lamp is used to decontaminate the internal chamber in between experiments.
Overhead White Light
The overhead white light can remain lit at any time throughout experiment procedures or the
decontamination process. The fluorescent white light provides a bright light within the PCR Hood. To
use the white light, press the ON button located on the top panel.
Operating the UV/Air Circulator
The UV/Air Circulator is built into the UV2 and
HEPA/UV3 PCR Hoods.
To operate the Circulator, press the UV/Air Circulator
switch to the ON position. The Circulator will pull air
from inside the chamber through the Circulator,
decontaminating the air with shortwave UV and
blowing the air back into the chamber.
NOTE: Do not place equipment in front of the air
vents, as this will block air flow.
An optional UV/Air Circulator module can be
mounted into the top of the UV1 PCR Workstation
(95-00367-XX only) for elimination of amplicons of
DNA or genomic DNA from a previously dispensed
experiment. The power switch, outlet, and mounting
brackets for the Circulator are pre-installed at the
factory if users purchase the Circulator at a later time.
Operating the HEPA/UV System (HEPA/UV3 Models Only)
Push the HEPA/UV power switch ON to operate the HEPA/UV filter system. The airflow fan setting can
be adjusted from Low to High, circulating anywhere from 22 to 50 feet per minute (FPM) of air. The filter
system consists of:
•
Pre-Filter to preserve the life of other filters by capturing large dust particles
•
Carbon Filter to remove ozone, gases, odor
•
HEPA Filter to provide a barrier (99.99%) against dust, bacteria and mold down to 0.3 micron
particles (NOTE: The HEPA filter does not prevent particulates from entering the workstation when
the Makrolon
® door is open).