Luminex xPONENT 4.2 for FLEXMAP 3D - Manual (Page 11)

Revive After Storage Routine
NOTE: The Revive After Storage routine is necessary when the system
has been idle for more than a week.
After you have adjusted the sample probe height, run the Revive After Storage (Luminex)
routine.
1. Open the Maintenance page, then the Cmds & Routines tab.
2. Select Revive After Storage (Luminex) from the Routine Name drop-down list. The
Revive After Storage routine performs the following commands:
• Warmup
• Backflush (x2)
• Drain RA2 (x3)
• Alcohol Flush RB1 (x2)
• Backflush
• Wash RA1 (x3)
3. Add 70% isopropanol or 70% ethanol to reservoir RB1 on the off-plate reagent block as
indicated on the Cmds & Routines tab. Add DI Water to reservoir RA1.
NOTE: The drain reservoir (RA2) should be empty.
4. Click Run.
After the Revive After Storage routine is complete, run the System Initialization routine.
Initialize the System
xPONENT ® enables you to customize system initialization using one of these three options:
• Laser warm-up, fluidics prep, calibration, performance verification
• Laser warm-up, fluidics prep, performance verification
• Warm-up fluidics prep
NOTE: Verify the system daily and calibrate weekly to check system integrity
and to ensure that calibration is still valid.
To run the system initialization routine:
1. On the Home page, click System Initialization.
2. Select the performance verification kit from the Performance Verification Kit list.
3. Select the calibration kit from the Calibration Kit list.
4. Vortex the xMAP calibrator, verification, and fluidics containers for 30 seconds to ensure
homogeneity. Do not dilute xMAP calibrator, verification, or fluidics agents.
5. Click Eject on the status bar.
6. Place the off-plate reagent block on the plate holder. Load a strip well into the block.
7