Adjusting the sample probe height – Luminex xPONENT 4.2 for FLEXMAP 3D User Manual
Page 9
Adjusting the Sample Probe Height
Adjust the sample probe height to ensure that the probe drops far enough into the well to
acquire sample.
NOTE: Ensure that there is no liquid in the wells or reservoirs before
adjusting the sample probe height.
NOTE: When you adjust and save the probe height settings for all three
areas under a plate name, all areas retain the adjustment.
WARNING:
Correct sample probe height is critical to successful sample
acquisition and calibration. Problems with the sample probe
height can lead to fluid leaks and inhibit sample acquisition.
1. On the Home page, click Probe and Heater under Daily Activities. The Probe &
Heater tab opens.
2. Select a plate in the Plate Type list.
• 96-well and 384-well Hard Bottom Plates - No disk is required. Ensure that the well
location is selected on the plate image. Use well D6 for the 96-well plate and well H12
for the 384-well plate (a green pin marks the location). To change the well location,
click on the desired well in the plate image.
• 96-well Filter or Mylar Bottom Plate - Place one (5.08) alignment disk into a well.
Ensure that the well location is selected on the plate image. Luminex
®
recommends
using well D6 for 96-well plates.
• 384-well Filter or Mylar Plate - Use the Probe Height Adjustment Tool (not supplied).
NOTE: You can order the Probe Height Adjustment Tool (P/N CN-0298-01)
3. Verify that the microtiter is not warped. Warped plates can lead to incorrect probe height
adjustment.
4. Click Eject to eject the plate carrier.
NOTE: If using a strip-well plate, ensure that a strip is present in the
selected well location, prior to calibration.
5. Place the plate on the plate holder with A1 in the top left corner. Place the off-plate
reagent block on the plate carrier. Place a well-strip (provided with the Calibration and
the Performance Verification kits) in column S1 of the block.
NOTE: Make sure there is no liquid in the plate or the off plate reagent
block.
6. Click Retract to retract the plate carrier.
5