Luminex xPONENT 4.2 for FLEXMAP 3D - Manual (Page 15)

You can create each quantitative, qualitative, and allele call protocols using one product
composed of a definable number of standards and controls.
There are three steps to creating a protocol. All three are accessible from the Protocols
page, Protocols tab. The three steps are:
• Define settings.
• Select the analysis type.
• Define the plate layout.
To create a protocol:
1. Open the Protocols page.
2. Click Create New Protocol. The Settings tab opens.
3. Type the protocol name, version, and manufacturer.
4. Type the following information in the Acquisition Settings panel:
• Sample volume (10-200μL)
• XY heater temperature (35-60°C)
• Timeout (1-250 seconds)
• Bead Type
• Plate name
• DD gating
• Reporter Gain
5. Select the analysis type (Qualitative, Quantitative, Allele Call, or None), and enter the
number of standards and controls.
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