Eppendorf Pipette Reference 2 - Manual (Page 16)

Operation
Eppendorf Reference
® 2
English (EN)
16
4.3 Deactivating the tip cone spring loading
For the following dispensing tasks, it may be helpful to deactivate the spring loading action of the tip cone.
• A pipette tip is to be used over a long period of time.
• The pipette tip is slightly bent during dispensing.
• For pipette tips that are not designed for the tip cone of Eppendorf pipettes.
The spring loading action of the tip cone on single-channel pipettes can be blocked by installing a locking
ring.
On multi-channel pipettes, the spring loading action of the tip cone can be switched on or off using the
selection switch.
4.4 Aspirating liquid
4.5 Dispensing liquid
1. The liquid to be aspirated must be taken from a suitable vessel. For multi-channel
pipettes, we recommend the reagent reservoir "Tip-Tub".
2. Press down the control button to the first stop (measuring stroke).
3. Immerse the pipette tip(s) vertically approx. 4
mm into the liquid.
4. To aspirate liquid, allow the control button to slide back slowly. Maintain the immersion
depth to ensure no air is aspirated.
5. In the case of large volumes: before removing the pipette tip from the liquid, wait for
approx. 3 seconds. To ensure maximum precision and accuracy, we recommend to wet
each new tip initially by aspirating and dispensing the liquid one to three times. Only
then should pipetting commence.
6. Remove the tip(s) slowly from the liquid.
7. Wipe the tip(s) slowly against the tube wall to ensure that no outer wetting remains on
the tip.
1. Place the tip on the tube inner wall at an angle.
2. Press the control button slowly until the first stop (measuring stroke) and wait until the
flow of liquid stops.
3. To empty the tip, press the control button until the second stop (blow-out).
4. Hold down the control button and wipe the tip on the tube inner wall.
5. To eject the tips, hold the control button all the way down.