Eppendorf Pipette Reference 2 - Manual (Page 23)

Maintenance
Eppendorf Reference ® 2
English (EN)
23
6.4.1.3 Opening and assembling the lower part (≤ 1000 μL)
To avoid confusion of parts, only disassemble and assemble one pipette at a time.
Opening the lower part:
1. Push the stop pins together slightly on the piston mount (1).
2. Remove the piston mount.
3. Remove the piston (2) and the piston spring (3). The piston is under
spring tension. The appearance of pistons and piston springs will
vary for the individual volume versions.
Assembling the lower part:
1. Carefully guide the piston and the piston spring into the cylinder.
Make sure that the piston is guided correctly in the piston spring and
the cylinder. There must not be any perceptible resistance.
Stop
pushing as soon as any resistance is felt. This may indicate that the
piston is not positioned correctly in the cylinder. There is a risk of
bending the piston if too much force is applied. Carefully pull out the
piston and repeat the process correctly. In the case of piston springs
with double windings (4), these windings must point downwards.
2. Keep the piston and the piston spring pressed.
3. Keep the stop pins on the piston mount pressed with the other hand.
4. Mount the piston mount so that the two locking mechanisms engage
in their retainers.
5. Gently press a pipette tip against the inserted piston. The piston must
move down in the cylinder without any noticeable resistance.
Installation of the lower part and ejector sleeve:
1. Insert the lower part into the upper part until it audibly engages.
2. Set the maximum volume and operate the control button several
times. It must run smoothly and resistance-free.
3. Keep the ejector pressed. The ejector rod protrudes from the upper
part.
4. Place the ejector sleeve on the ejector rod. If correctly fitted, it will
engage slightly.
5. Carry out a gravimetric test of the systematic and random error.
This test ensures that no parts were confused during assembly and that
the pipette has been assembled correctly.
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