Eppendorf Pipette Reference 2 - Manual (Page 20)

Maintenance
Eppendorf Reference
® 2
English (EN)
20
6.2 Sterilizing or disinfecting the pipette
6.2.1 Autoclaving
All Reference 2 pipettes are completely steam autoclavable.
6.2.1.1 Before autoclaving
 Remove any contamination from the exterior and the lower part.
 If you remove existing grease, slightly re-lubricate the piston seal using only the grease specified in the
ordering information (see the enclosed CD).
6.2.1.2 Procedure
Prerequisites
• Follow the operating manual of the autoclave manufacturer.
• Autoclave at: 121 °C, 20 minutes, 1 bar overpressure.
1. When placing the Reference 2 into the autoclave, make sure that the temperature at the pipette does not
exceed 121 °C.
2. You can put the Reference 2 into the autoclave as a complete unit or with the lower part removed. Do
not disassemble the lower part.
3. For 2 mL to 10 mL pipettes: remove old protection filter. Add a filter sleeve and new protection filter and
insert them after autoclaving. Only autoclave the protection filter once.
4. If lower parts have been removed, make sure that no lower parts are confused during reassembly. (Tip:
use one plastic beaker per pipette.)
6.2.1.3 After autoclaving
 Cool the pipette to room temperature and let it dry.
 For 2 mL to 10 mL pipettes: press the protection filter into the filter sleeve and insert it into the tip cone.
The piston does not need to be relubricated after autoclaving. If autoclaving is carried out as described
above, no gravimetric testing or adjustment is necessary.
NOTICE! Damage to device from incorrect handling.
 Do not use any additional disinfectants, decontamination agents or sodium hypochlorite
during autoclaving or UV exposure.
 When autoclaving, please ensure that the temperature does not exceed 121°C.
 Before using disinfecting agents or decontaminating agents, test for material compatibility
and check the manufacturer's instructions on chemical resistance. Please also note the
pipette material.