C.B.S. Scientific SG Series - Manual (Page 10)

C.B.S. œ Scientific
10
Adjustable Height Sequencer
3.3 Glass Plate Preparation
1. Select a pair of glass plates (if using a notched set, one notched and one unnotched back
plate) for the appropriate gel height. Be sure the edges of the plates are nick or chip free.
2. It is vital that these plates are absolutely CLEAN ! Wearing gloves (and rinsing off any
residual powder), clean the glass plates thoroughly with a non abrasive detergent and a
plastic scouring pad. The cleaning solution should not leave a soap residue when rinsed
thoroughly.
3. Rinse the glass plates thoroughly with de ionized water and wipe dry.
4. If desired, treat the inside (acrylamide contact) surface of one (usually the notched plate) or
both glass plates with a silanizing reagent, such as C.B.S. Scientific's Gel Repel, following
the manufacturer's instructions.
5. Immediately before assembling the glass plate 'sandwich', rinse the inside surfaces with 95%
ethanol and wipe dry with a lint-free paper towel. Avoid touching the inside surfaces with
your fingers. The wearing of gloves is highly recommended. Impeccably clean, dust free
plates are essential for pouring gels without bubbles.
6. Assemble the glass plate sandwich in the conventional manner; place the back glass plate
inside-up on the bench, align the cleaned spacers at the sides, and place the notched plate
inside-down on the side spacers.