C.B.S. Scientific SG Series - Manual (Page 15)

C.B.S. œ Scientific
15
Adjustable Height Sequencer
Section 4
Electrophoresis
4.1 Pre-electrophoresis
1. Remove the gel sealing tape from the bottom of the gel sandwich, if tape was used. Rest the
bottom edge of the sandwich on the footing in the lower reservoir, notched plate inward.
Using mounting clamps (Cat.# GPC-0001) attach the lower section of the glass plate
assembly to the upright of the sequencer. Do not clamp glass plate assembly to the upper
reservoir until the sharktooth comb is inserted.
2. If you are using a sharktooth comb, insert the comb (teeth-down) between the glass plates.
Insert the comb until the teeth just make contact with the surface of the gel. Do NOT allow
the teeth to pierce the gel. A very slight indentation of the gel should be visible when the
comb is properly inserted. Do not slide the comb laterally after it has come in contact with
the gel.
3. Slide the upper reservoir to the glass plate sandwich and clamp using the bar clamp. Pre-
loosen black wing nuts so clamp assembly slides over glass plates and upper reservoir,
without disturbing the comb. Tighten wing nuts only enough to compress gasket. Do NOT
over tighten. See figures below.
4. Mount the aluminum plate to the back side of the glass plate sandwich using GPC-0001
clamps. Be sure the aluminum plate does not go in the lower buffer chamber.