UVP BioChemi System with EpiChemi II Darkroom - Manual (Page 25)

C. Chemiluminescent samples (Films and Membranes/Plates)
Emitted light in a light tight environment.
• The time to capture chemiluminescence with the BioChemi system will
depend on several factors, but typically is equivalent to the time it would take
for the same sample on film.
• Always make sure to start integration immediately after the substrate has
been added, especially since some substrates can degrade quickly (we
recommend BioWest Extended Duration substrate).
1. Move filter wheel to the Clear position.
2. Open LabWorks software.
3. Pull down black sample tray and place UVP target or printed text on
the tray.
4. Reset focus.
5. Turn overhead light off.
6. Place sample on the black tray.
7. Set the zoom by turning the lower portion of the lens all the way to
the right.
8. Click on the Camera Icon.
9.
The Minimal Dialog window will display. Click Stop/Capt. Button.
Stop/Capt Button
The Options button will become available.
Options Button
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