UVP BioChemi System with EpiChemi II Darkroom - Manual (Page 31)

Dynamic Integration Auto Exposure – Automatic accumulated exposure
of multiple on-chip integrations before image saturation.
Application: Immunoblot protocols using enhanced luminol substrates with
high sensitivity and extended duration (BioWest, SuperSignal)
1. With the Integration tab open, check the Dynamic
Integration, Auto Exposure, and Saturation Warning
boxes.
2. Enter the amount of time you want to expose each frame.
Each frame will be added to the one before it in an
arithmetic progression.
3. Click Capture. This automatically captures any live image
before the brightest pixel reaches saturation.
4. For longer exposures, it is best to also check the
Integration box and set the seconds for approximately 1/10
of the total expected exposure time (i.e. 10 minutes = 60
seconds). This allows the CCD chip to be read every
interval selected, displaying the image as it exposes over
the entire integration time.
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